Cladocerans are used extensively
internationally for aquatic toxicity testing and have been found
to adapt well to laboratory conditions (Environment
Canada 1992; USEPA
1993; Hall
and Golding 1998). The functional role of
cladocerans in a freshwater community favours them as test animals. They have
an important role in converting phytoplankton and bacteria into
nutritionally valuable food for species higher up the food chain.
Acute and chronic test
methodologies have been developed (e.g. Environment
Canada 1992; ASTM
1997) and applied to both pure
chemical and industrial wastewater discharges (USEPA 1985). The chronic
three-brood test method was introduced for use in effluent and
ambient water evaluations, and is one of several methods used by
the USEPA and Environment Canada for regulatory monitoring
programs. |
Ceriodaphnia
dubia
|
Acute 48h survival test
Juveniles (<24h old) are exposed to
various concentrations of a test substance under defined conditions. The
survival of the organisms after 48h exposure is compared to the survival
of the organisms in an appropriate control. A test substance is
considered toxic when a statistically significant, dose-dependent effect
on survival of the organisms is observed.
Chronic 7d survival and reproduction test
Juveniles (<24h old) are exposed to
various concentrations of a test substance in a static-renewal test
system for a minimum of 7days. The survival of the exposed organisms is
compared to the survival in an appropriate control. The number of
offspring produced in three broods during this exposure for different
concentrations of the test substance is compared to the number of
offspring produced during exposure to an appropriate control.
Paracalliope
fluviatalis
|
The freshwater amphipod Paracalliope
fluviatilis is potentially one of the most useful indigenous
freshwater species for toxicity testing. It is common throughout
New Zealand and usually found in flowing waters, inhabiting
sediment and weed beds along the edges of streams. Previous
studies have found them to be sensitive to a wide range of
contaminants (e.g Burnett
1972; Hunt
1974; Hickey
and Vickers 1994; Hall and Golding 1998) and their relative sensitivity suggests that P.
fluviatilis may be one of the most vulnerable species to
contaminants. |
Acute 48h survival test
Juveniles aged between 0 and 7 days
are exposed in a static system to various concentrations of a test
substance for 48h. The survival of the amphipods exposed to the test
substance is compared to the survival of the amphipods in an appropriate
control. A test substance is considered toxic when a statistically
significant, dose-dependent effect on amphipod survival occurs.
Chronic 30d survival and reproduction test
Juveniles between 1-3mm length are exposed in a flow
through test system to various concentrations of a test substance for 30
days. At the completion of the test exposure, the number of surviving
adults and juveniles are counted and compared to the number of adults
and juveniles in an appropriate control.
|