New Zealand ecotoxicity data for soil invertebrates

Test organism	Test concentrations mg/kg^a	End point	NOEC	LOEC	EC₅₀ or LC₅₀
_{Earthworm Aporrectodea caliginosa}	_{Cu (II) {0, 100, 200, 300, 400, 600}}	_{14 day growth (adult)}	_<₁₀₀	₁₀₀	₉₉ _[78,127]
	_{Cu (II) {0, 100, 200, 300, 400, 600}}	_{28 day growth (adult)}	₁₀₀	₂₀₀	₁₁₄ _[72,181]
	_{Cu (II) {0, 150, 200, 250, 300}}	_{14 day growth (juvenile)}	_<₁₅₀	₁₅₀	₁₂₅ _[104,151]
	_{Cu (II) {0, 150, 200, 250, 300}}	_{28 day growth (juvenile)}	_<₁₅₀	₁₅₀	₁₂₇ _[106,154]
_{Woodlice Porcellio scaber}	_{Cu (II) {0, 1200, 1600, 2000, 2400}}	_{14 day growth _(juvenile)}	₂₄₀₀	_>2400	_{No effect up to 2400 mg/kg}
	_{Cu (II) {0, 1200, 1600, 2000, 2400}}	_{28 day growth _(juvenile)}	₂₄₀₀	_>2400	_{No effect up to 2400 mg/kg}
	_{Cu (II) {0, 1200, 1600, 2000, 2400}}	_{14 day mortality _(juvenile)}	₂₄₀₀	_>2400	_{No effect up to 2400 mg/kg}
	_{Cu (II) {0, 1200, 1600, 2000, 2400}}	_{28 day mortality _(juvenile)}	₂₄₀₀	_>2400	_{No effect up to 2400 mg/kg}

^a Nominal test concentrations in mg/kg are given inside { }

NOEC and LOEC were calculated using one-way analysis of variance (ANOVA), and post hoc Dunnett’s test at a significance level of p < 0.05. To fit assumption of normality for an ANOVA, percentage data was arcsin transformed, square root or natural log transformations were applied to other data where appropriate, prior to analysis.

LC₅₀ values were derived from probit analysis, 95% confidence intervals are given in [ ].

EC₅₀ values and their 95% confidence intervals were estimated by fitting logistical models to the data using non-linear regression. Where necessary, weighted regression was performed to allow for heteroscedasticity in the data.

Chromium

Test organism	Test concentrations mg/kg^a	End point	NOEC	LOEC	EC₅₀ or LC₅₀
_{Earthworm Aporrectodea caliginosa}	_{Cr (VI) {0, 150, 200, 250, 300, 400}}	_{14 day growth (adult)}	_<₁₅₀	₁₅₀	_**
	_{Cr (VI) {0, 150, 200, 250, 300, 400}}	_{28 day growth (adult)}	_<150	₁₅₀	_**
	_{Cr (VI) {0, 50, 100, 200, 300, 400}}	_{14 day growth (juvenile)}	_<50	₅₀	_**
	_{Cr (VI) {0, 50, 100, 200, 300, 400}}	_{28 day growth (juvenile)}	_<50	₅₀	₁₀₁ [17, 587]
_{Woodlice Porcellio scaber}	_{Cr (IV) {0, 300, 400, 500, 600}}	_{14 day growth _(juvenile)}	₆₀₀	_>600	_{No effect up to 600 mg/kg}
	_{Cr (IV) {0, 300, 400, 500, 600}}	_{28 day growth _(juvenile)}	₆₀₀	_>600	_{No effect up to 600 mg/kg}
	_{Cr (IV) {0, 300, 400, 500, 600}}	_{14 day mortality _(juvenile)}	₄₀₀	₅₀₀	_{544 [481-693]}
	_{Cr (IV) {0, 300, 400, 500, 600}}	_{28 day mortality _(juvenile)}	₃₀₀	₄₀₀	_{428 [386-472]}

^a Nominal test concentrations in mg/kg are given inside { }

LC₅₀ values were derived from probit analysis, 95% confidence intervals are given in [ ].

** EC₅₀ not derived where toxicity curve was incomplete or did not follow a dose-response relationship.

Arsenic

Test organism	Test concentrations mg/kg^a	End point	NOEC	LOEC	EC₅₀ or LC₅₀
_{Earthworm Aporrectodea caliginosa}	_{As (V) {0, 50, 100, 200, 300, 400}}	_{14 day growth (adult)}	₅₀	₁₀₀	₅₈ _[47,71]
	_{As (V) {0, 50, 100, 200, 300, 400}}	_{28 day growth (adult)}	₅₀	₁₀₀	₄₅ _[20,102
	_{As (V) {0, 5, 10, 20, 40, 60, 80, 100}}	_{14 day growth (juvenile)}	₄₀	₆₀	_**
	_{As (V) {0, 5, 10, 20, 40, 60, 80, 100}}	_{28 day growth (juvenile)}	40	60	**
	_{As (V) {0, 100, 200, 300, 400, 600}}	_{14 day mortality (juvenile)}	₄₀₀	₆₀₀	₅₅₂
	_{As (V) {0, 100, 200, 300, 400, 600}}	_{28 day mortality (juvenile)}	₂₀₀	₃₀₀	₅₂₂
_{Woodlice Porcellio scaber}	_{As (V) {0, 50, 100, 200, 300, 400}}	_{14 day growth _(juvenile)}	₄₀₀	_>400	_{No effect up to 400 mg/kg}
	_{As (V) {0, 50, 100, 200, 300, 400}}	_{28 day growth _(juvenile)}	₄₀₀	_>400	_{No effect up to 400 mg/kg}
	_{As (V) {0, 50, 100, 200, 300, 400}}	_{14 day mortality _(juvenile)}	₂₀₀	₃₀₀	_{207 [152, 285]}
	_{As (V) {0, 50, 100, 200, 300, 400}}	_{28 day mortality _(juvenile)}	₂₀₀	₃₀₀	_{241 [186, 282]}

^a Nominal test concentrations in mg/kg are given inside { }

LC₅₀ values were derived from probit analysis, 95% confidence intervals are given in [ ].

** EC₅₀ not derived where toxicity curve was incomplete or did not follow a dose-response relationship.

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Page last updated: 01 May 2007