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New Zealand ecotoxicity data for terrestrial plants

Ecotoxicity testing protocols 

Ecotoxicity results

Soil micro-organisms

Soil invertebrates

Plants

Freshwater invertebrates

Freshwater fish

Contaminant mobility

Soil property data

Models

Toxicity databases

RA methodologies

Document resources

 

Problem Identification

Receptor Characterisation

Exposure Assessment

Toxicity Assessment

Risk Characterisation

Liability and use of this information - Please read

Copper

Species

Test concentration mg/kga

Test Duration

Test

End point 

NOEC

LOEC

EC50 or LC50

Lettuce

Lactuca sativa

 

 

 

 

 

 

Cu (II)

{0,600,1200,

1800,2400}

2 weeks

Seed germination & viability

increased time to emergence

2400

>2400

Not applicable

decreased germination

2400

>2400

**

decreased viabilityb

1800

2400

**

Cu (II)

{0,62.5,125,250,

500,1000, 2000}

2 weeks

Seedling growthc

decreased root weight

 

500

 

1000

 

EC50 = 750

 [560, 1010]

decreased shoot weight 250 500

EC50 = 500

 [390, 630]

 

seedling mortality

2400

>2400

**

Cu (II)

{0,600,1200,

1800,2400}

2 weeks Seedling growthc

Millet

Panicum milliaceum

 

Cu (II)

{0,600,1200,

1800, 2400}

2 weeks

Seed germination & viability

increased time to emergence

>2400

>2400

Not applicable

decreased germination

1800

2400

**

decreased viabilityb 1800 2400 **

Seedling growthc

 

 

decreased root weight

600

1200

EC50=1800 

[970, 3630]

decreased root length

<600 600 EC50=310

[190, 500]

decreased shoot weight 600 1200 EC50=1320

[1030, 1690]

seedling mortality 2400 > 2400 **

Kakabeak

Clianthus puniceus

 

 

 

 

 

 

Cu (II) {0, 600, 1200, 1800, 2400} 4 weeks Seed germination & viability increased time to emergence 1200 1800 **

decreased germination

1800 2400 **
decreased viabilityb 1800 2400 **
Seedling growthc

decreased root weight

1200 1800 EC50=1380

[1010, 1890]

decreased root length

< 600 600 EC50=640

[570, 730]

decreased shoot weight 600 1200 EC50=1180

[900, 1590]

seedling mortality 2400 > 2400 **

a nominal test concentrations in mg/kg are given in {}.

b Seed viability is a measure which includes % germination as well the survivability of germinated seedlings (seed viability = % germinated x % seedling survival).

c Seedling growth is measured at the end of test duration i.e. 2 or 4 weeks after seeds were sown.

NOEC and LOEC were calculated using one-way analysis of variance (ANOVA), and post hoc Dunnett�s test at a significance level of p < 0.05. To fit assumption of normality for an ANOVA, percentage data was arcsin transformed, square root or natural log transformations were applied to other data where appropriate, prior to analysis.

LC50 values were derived from probit analysis, 95% confidence intervals are given in [ ].

EC50 values and their 95% confidence intervals were estimated by fitting logistical models to the data using non-linear regression.  Where necessary, weighted regression was performed to allow for heteroscedasticity in the data.

** EC50 not derived where toxicity curve was incomplete or did not follow a dose-response relationship.

Chromium

Species

Test concentration mg/kga

Test Duration

Test

End point

NOEC

LOEC

EC50 or LC50

Lettuce

Lactuca sativa

 

 

 

 

 

 

Cr (VI)

{0, 20, 40, 60, 80}

 

 

 

 

 

 

2 weeks

 

 

 

 

 

 

Seed germination & viability

 

 

increased time to emergence

20

40

Not

applicable

decreased germination

20

40

**

decreased viabilityb

20

40

LC50 = 45

 [30, 60]

Seedling growthc

 

 

 

decreased root weight

<20

20

EC50 = 19

 [15, 24]

decreased root length

20

40

EC50 = 28

 [24, 33]

decreased shoot weight

<20

20

EC50 = 17

 [12, 24]

seedling mortality

60

80

LC50 = 50

 [37, 68]

Millet

Panicum milliaceum

 

 

 

 

 

 

Cr (VI)

{0, 20, 40, 60, 80}

 

 

 

 

 

 

2 weeks

 

 

 

 

 

 

Seed germination & viability

 

 

increased time to emergence

80

>80

Not

applicable

decreased germination

80

>80

**

decreased viabilityb

80

>80

**

Seedling growthc

 

 

 

decreased root weight

80

>80

**

decreased root length

40

60

EC50 = 69

 [59, 81]

decreased shoot weight

40

60

EC50 = 60

 [47, 77]

seedling mortality

80

>80

**

Kakabeak

Clianthus puniceus

 

 

 

 

 

 

Cr (VI)

{0, 20, 40, 60, 80}

 

 

 

 

 

 

4 weeks

 

 

 

 

 

 

Seed germination & viability

 

 

increased time to emergence

<20

20

Not

applicable

decreased germination

60

80

**

decreased viabilityb

80

>80

**

Seedling growthc

 

 

 

decreased root weight

<20

20

EC50 = 44

[31, 63]

decreased root length

20

40

EC50 = 65

 [63, 68]

decreased shoot weight

20

40

EC50 = 48

 [39, 58]

seedling mortality

80

>80

**

a nominal test concentrations in mg/kg are given in {}.

b Seed viability is a measure which includes % germination as well the survivability of germinated seedlings (seed viability = % germinated x % seedling survival).

c Seedling growth is measured at the end of test duration i.e. 2 or 4 weeks after seeds were sown.

NOEC and LOEC were calculated using one-way analysis of variance (ANOVA), and post hoc Dunnett�s test at a significance level of p < 0.05. To fit assumption of normality for an ANOVA, percentage data was arcsin transformed, square root or natural log transformations were applied to other data where appropriate, prior to analysis.

LC50 values were derived from probit analysis, 95% confidence intervals are given in [ ].

EC50 values and their 95% confidence intervals were estimated by fitting logistical models to the data using non-linear regression.  Where necessary, weighted regression was performed to allow for heteroscedasticity in the data.

** EC50 not derived where toxicity curve was incomplete or did not follow a dose-response relationship.

Arsenic

Species

Test concentration mg/kga

Test Duration

Test

End point 

NOEC

LOEC

EC50 or LC50

Lettuce

Lactuca sativa

 

 

 

 

 

As (V)

{0,100,200, 275,350,400}

 

2 weeks

Seed germination & viability

 

 

increased time to emergence

275

350

Not applicable

decreased germination

400

>400

**

decreased viabilityb

275

350

LC50 = 310

 [260, 390]

As (V)

{0,25,50,100,200}

 

2 weeks

Seedling growthc

 

decreased root weight

50

100

EC50 = 65

 [51, 83]

decreased root length

50

100

EC50 = 110

 [98, 124]

decreased shoot weight

25

50

EC50 = 60

 [39, 92]

As (V)

{0,100,200, 275,350,400}

   

seedling mortality

275

350

LC50 = 340

 [320, 360]

Millet

Panicum milliaceum

 

 

 

 

 

 

 

 

As (V)

{0,100,200, 275,350,400}

 

 

 

 

 

 

2 weeks

 

 

 

 

 

 

Seed germination & viability

 

 

increased time to emergence

100

200

Not applicable

decreased germination

400

>400

**

decreased viabilityb

400

>400

**

Seedling growthc

 

 

 

decreased root weight

<100

100

**

decreased root length

<100

100

EC50 = 65

 [45, 93]

decreased shoot weight

<100

100

EC50 = 29

 [13, 61]

seedling mortality

350

400

**

Kakabeak

Clianthus puniceus

 

 

 

 

 

 

As (V)

{0,100,200, 275,350,400}

 

 

 

 

 

 

4 weeks

 

 

 

 

 

 

Seed germination & viability

 

 

increased time to emergence

 

 

 

decreased germination

 

 

 

decreased viabilityb

 

 

 

Seedling growthc

 

 

 

 

decreased root weight

 

 

 

decreased root length

 

 

 

decreased shoot weight

 

 

 

seedling mortality

 

 

 

a nominal test concentrations in mg/kg are given in {}.

b Seed viability is a measure which includes % germination as well the survivability of germinated seedlings (seed viability = % germinated x % seedling survival).

c Seedling growth is measured at the end of test duration i.e. 2 or 4 weeks after seeds were sown.

NOEC and LOEC were calculated using one-way analysis of variance (ANOVA), and post hoc Dunnett�s test at a significance level of p < 0.05. To fit assumption of normality for an ANOVA, percentage data was arcsin transformed, square root or natural log transformations were applied to other data where appropriate, prior to analysis.

LC50 values were derived from probit analysis, 95% confidence intervals are given in [ ].

EC50 values and their 95% confidence intervals were estimated by fitting logistical models to the data using non-linear regression.  Where necessary, weighted regression was performed to allow for heteroscedasticity in the data.

** EC50 not derived where toxicity curve was incomplete or did not follow a dose-response relationship.

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